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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 年限：
3.5 days embryo, blastocyst
- ATCC Number：
SCRC-1036™
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
胚胎干细胞
- 细胞形态：
球形
- 库存：
大量
- 组织来源：
inner cell mass
- 器官来源：
胚胎
- 品系：
129X1 x 129S1
| Designations: | R1/E | ||
| Depositors: | A Nagy | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | spherical colony |
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| Source: | Strain: 129X1 x 129S1 Organ: embryo Tissue: inner cell mass Cell Type: embryonic stem cell; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Prior to purchase, for-profit commercial institutions must obtain a license agreement. For instructions on how to proceed, please contact ATCC 's Office of Licensing and Business Development at licensing@ATCC .org or 703 365 2773. | ||
| Applications: | The segregation could result in several coat types, from albino, through light brown, to black, depending on the genetic background of the partner of the germline chimaera. The R1 cell line was established in August 1991, from a 129X1 x 129S1 3.5 day blastocyst. No live offspring were produced from cells older than passage #14. However, about 20% of subclones derived from passage #14 had the original developmental potential of R1 when tested by tetraploid aggregates [PubMed: 8378314]. Pluripotency of R1 was initially tested by tetraploid embryo <-> ES aggregates for completely ES derived development [PubMed: 8378314]. |
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| Age: | 3.5 days embryo, blastocyst | ||
| Gender: | male | ||
| Comments: | The R1/E cell line was subcloned from R1 in EMBL, Heidelberg, Germany by Kristina Vintersten. The R1 cell line was established in August 1991, from a 129X1 x 129S1 3.5 day blastocyst. The cells are heterozygous for the c locus (+/c (ch)) and for the pink eye locus (+/p). This mouse ES cell line has been shown to be germline competent.In the F1 generation the coat color is uniform agouti, while in the F2 these two coat color genes segregate. The segregation could result in several coat types, from albino, through light brown, to black, depending on the genetic background of the partner of the germline chimaera. Pluripotency of R1 was initially tested by tetraploid embryo <-> ES aggregates for completely ES derived development [PubMed: 8378314]. They were also tested by diploid embryo <-> ES aggregates and blastocyst injection for germline transmission in chimeras [PubMed: 8361547]. At early passages (up to passage #14), one third of the completely R1-derived newborns generated by tetraploid embryo <-> R1 aggregates survived. No live offspring were produced from cells older than passage #14. However, about 20% of subclones derived from passage #14 had the original developmental potential of R1 when tested by tetraploid aggregates [PubMed: 8378314]. R1-derived animals reached adulthood and were fertile. The genetically altered lines derived from R1 gave high efficiency of germline transmission either by injecting them into C57 blastocyst or aggregating them with CD-1 or ICR outbred 8-cell stage embryos. More than 90% of the individual K.O. clones went to germline (n>60) by aggregation chimeras. |
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| Propagation: | ATCC complete growth medium: ES-DMEM (ATCC SCRR-2010) supplemented with 2.0 mM L-Alanyl-L-Glutamine (ATCC 30-2115), 0.1 mM non-essential Amino Acids (ATCC 30-2116), 0.1 mM 2-mercaptoethanol (Invitrogen Life Technologies No. 21985), 1000 U/ml mouse leukemia inhibitory factor (LIF) (Chemicon No. ESG1107) and 15% fetal bovine serum (ATCC SCRR-30-2020). Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Establishing and maintaining your culture:To insure the highest level of viability, be sure to warm media to 37�C before using it on the cells.
Interval: Every one to two days Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:7 is recommended. Medium Renewal: Every day |
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| Preservation: | Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 10% DMSO (ATCC 4-X ). Storage temperature: liquid niitrogen vapor phase |
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| Related Products: | parental cell line:ATCC SCRC-1011 | ||
| References: | 57459: Matise M, et alProduction of targeted embryonic stem cell clonesIn: Matise M, et alGene Targeting: A Practical ApproachOxfordOxford University Press101-132, 1999 71506: Nagy A, et al. Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc. Natl. Acad. Sci. USA : 8424-8428, 1993. PubMed: 8378314 71870: Wood SA, et al. Non-injection methods for the production of embryonic stem cell-embryo chimaeras. Nature 365: 87-89, 1993. PubMed: 8361547 71871: Nagy A, Rossant JProduction and analysis of ES-cell aggregation chimerasIn: Nagy A, Rossant JGene Targeting: A Practical ApproachOxfordOxford University Press177-206, 1999 |
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R1/E
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