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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 器官来源：
肺
- 运输方式：
冻存运输
- 相关疾病：
其他疾病
- 库存：
大量
- ATCC Number：
CRL-1848™
- 年限：
32 years
| Designations: | NCI-H292 [H292] | ||
| Depositors: | AF Gazdar | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: mucoepidermoid pulmonary carcinoma |
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| Cellular Products: | keratin; vimentin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein. The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase. This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. |
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| Virus Susceptibility: | Hepatitis B virus | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 9,13 D5S818: 13 D7S820: 10 THO1: 8 TPOX: 8,11 vWA: 16,17 |
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| Cytogenetic Analysis: | This is a human cell line with near-diploid chromosome counts. The modal chromosome number was 47, occurring in 36% of cells. The rate of cells with a higher ploidy count was 3.9%. Twelve markers were common to most cells. Among them were del(1) (q32.1), der (5)t(5;13) (p15.33;q11), i(5p), der(1)t(1;?) (p34.3;?) and der (6)t(6;7) (p25.3;q21.2). All markers were present in single copy per cell. Normal N1 and N6 were absent. There were two normal X chromosomes. No other abnormalities were detected. | ||
| Age: | 32 years | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| Comments: | This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. The cells were isolated in a chemically defined medium (HITES) and later adapted to growth in media supplemented with serum. The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase. The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 48 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 22946: Yoakum GH, et al. High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells. Science 222: 385-389, 1983. PubMed: 6194563 23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257 |
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