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- 详细信息
- 技术资料
- 年限：
46 years
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
宫颈
- 库存：
大量
- 相关疾病：
鳞状细胞癌
- ATCC Number：
CRL-10302™
| Designations: | SW756 | ||
| Depositors: | Univ. Texas System, Board of Regents | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: cervix Disease: squamous cell carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Temple Texas, United States Isolation date: 1974 |
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| Virus Susceptibility: | Influenza A virus | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5 | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 11 D16S539: 12,13 D5S818: 11,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 17 |
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| Cytogenetic Analysis: | modal number = 76; range = 60 to 96 | ||
| Isoenzymes: | G6PD, B GLO-I, 1-2 |
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| Age: | 46 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| References: | 22075: Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995 22667: Freedman RS, et al. Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487 |
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SW756
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