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文献和实验AML Sample genomic DNA Preparation
Solutions PBS Cell lysis buffer: 10 mM Tris pH8, 100 mM NaCl, 25 mM EDTA, 0.5% (w/v) SDS, 0.5 mg/mL proteinase K Fixative: 1 volume glacial acetic acid + 3 volumes methanol Trizol reagent 75% EtOH made with RNAse free H2 O 100
Classification of AML by DNA-Oligonucleotide Microarrays
Accurate diagnosis and classification of leukemias are the bases for the appropriate management of patients. The diagnostic accuracy and efficiency of present methods may be improved by the use of microarrays. The followin g chapter gives
Classification of AML Using a Monoclonal Antibody Microarray
A cluster of differentiation (CD) antibody microarray called the DotScan microarray has been developed that enables an extensive immunophenotype to be obtained for a suspension of leukocytes in a single analysis. For a leukemia
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