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- 2026年01月14日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 器官来源：
肺
- 细胞形态：
上皮样
- 年限：
50 years
- 库存：
大量
- 生长状态：
贴壁生长
- 相关疾病：
小细胞肺癌
- ATCC Number：
CCL-257™
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | NCI-H1688 [H1688] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: carcinoma; classic small cell lung cancer |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 8 D16S539: 13 D5S818: 10 D7S820: 12 THO1: 6,8 TPOX: 11 vWA: 17 |
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| Age: | 50 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line was derived from a liver metastasis taken from a patient prior to therapy. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature. Remove the trypsin and incubate the flask at 37C for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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文献和实验一、实验材料NCI-H1299，购自上海晶莱生物技术有限公司。表 2.1.1 主要试剂试剂与耗材厂家（货号）细胞培养瓶FALCON 中国（353014）Penicillin/streptomycin solution（KGY002）0.25% Tripsin-EDTA（BK-E3076）PBS（BK330-2）胎牛血清GIBCO 美国（10270-106）1640 培养基Hyclone 美国（SH3080901）Annexin V-FITC/PI 凋亡试剂盒 二、实验仪器表 2.2.1 主要
2017 年 8 月 31 日，山东烟台毓璜顶医院呼吸内科邹慎春为第一作者和通讯作者 Yao Zhang 在《分子医学报告》（Molecular Medicine Reports）发表题为《Epicatechin induces apoptotic and autophagic cell death in NCI-H2172 non‑ small cell lung cancer cells along with concomitant mitochondrial membrane
Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs
into the lung cancer cell line NCI-H1299. The CPT–BB conjugate was metabolized by NCI-H1299 intracellular enzymes releasing the cytotoxic CPT. Receptor binding methods and fluorescent methods are presented for studying the internalization
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