SW 1990

SW 1990

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  • 2025年08月23日
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    • 详细信息
    • 询价记录
    • 技术资料
    • 生长状态

      贴壁生长

    • 库存

      大量

    • 年限

      56 years

    • 运输方式

      冻存运输

    • 器官来源

      胰腺

    • 细胞形态

      上皮样

    • 相关疾病

      腺癌

    • ATCC Number

      CRL-2172™

    • 物种来源

    • 是否是肿瘤细胞

      1

    Designations: SW 1990 [SW-1990, SW1990]
    Depositors:  W McCombs
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: epithelial

    Source: Organ: pancreas
    Disease: adenocarcinoma
    Derived from metastatic site: spleen
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Restrictions: These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432
    Isolation: Isolation date: 1978
    Tumorigenic: Yes
    Antigen Expression: Blood Type A; Rh +
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 10, 12
    D13S317: 8, 12
    D16S539: 13
    D5S818: 12, 13
    D7S820: 9, 10
    THO1: 9.3
    TPOX: 8, 9
    vWA: 17
    Cytogenetic Analysis: near triploid; range = 67 to 75
    Age: 56 years
    Gender: male
    Ethnicity: Caucasian
    Comments: The SW 1990 line was established in 1978 from a spleen metastasis of a grade II pancreatic adenocarcinoma deriving from the exocrine pancreas.
    The cells have a reported plating efficiency of 29%.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Atmosphere: air, 100%
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37�C.

        Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
        Medium Renewal: 2 to 3 times per week
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time: 64 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
    recommended serum:ATCC 30-2020
    References: 23047: Kyriazis AP, et al. Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse. Cancer Res. 43: 4393-4401, 1983. PubMed: 6871872

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