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- 详细信息
- 文献和实验
- 技术资料
- 器官来源：
乳房
- 相关疾病：
导管癌
- ATCC Number：
CRL-2322™
- 年限：
TNM stage IIA, grade 3
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 组织来源：
duct
- 库存：
大量
- 生长状态：
混合型生长
- 运输方式：
冻存运输
| Designations: | HCC1187 | ||
| Depositors: | AF Gazdar, AK Virmani | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed, adherent and suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: mammary gland; breast Tissue: duct Tumor Stage: TNM stage IIA, grade 3 Disease: primary ductal carcinoma |
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| Cellular Products: | Epithelial glycoprotein 2 (EGP2) cytokeratin 19 |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935. | ||
| Applications: | HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of Her2-neu; they overexpress p53. The cells are negative for expression of progesterone receptor (PR). The cells are poorly differentiated. |
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| Receptors: | progesterone receptor, negative | ||
| Oncogene: | her2/neu -, p53 + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 10 D5S818: 12 D7S820: 8,11 THO1: 6 TPOX: 8 vWA: 19 |
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| Cytogenetic Analysis: | multiploid; cell population has several ploidy indices | ||
| Age: | 41 years adult | ||
| Gender: | female | ||
| Ethnicity: | Caucasian, White | ||
| Comments: | This cell line was initiated on 9/13/94 from a patient who had received prior chemotherapy. The cell line took 4.5 months to establish. The tumor was classified as TNM stage IIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available. The cells are poorly differentiated. The cells are negative for expression of Her2-neu; they overexpress p53. HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of progesterone receptor (PR). An EBV transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323 . |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Attached cells may be subcultured using 0.25% trypsin-0.53 mM EDTA. Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 normal (or near-normal) cell line established from the same patient:ATCC CRL-2323 purified DNA:ATCC CRL-2322D purified RNA:ATCC CRL-2322R |
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| References: | 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 | ||
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文献和实验相关实验
Direct DNA Injection (p53) into HCC Tumors
Liver tumors, specially HCC, are among the most common malignancies in the world, and their annual world incidence is about 250,000 cases, with a male to female ratio 4:1 (1 ). HCC is one of the most important neoplasms in tropical
文献速递：全面液体分析循环肿瘤DNA和蛋白质生物标志物为评估预后带来新的可能
研究背景 肝细胞癌（HCC）被认为是中国癌症相关死亡率的第三大原因。尽管手术是治疗HCC的较佳选择，但由于缺乏早期症状，大多数患者在癌症晚期才被诊断出来，因此肿瘤复发的风险很高。识别可能复发的患者并给予对应的辅助治疗，仍然是一个临床难题。循环肿瘤DNA（ctDNA），也可称为肿瘤衍生的细胞游离DNA，包含有关肿瘤基因组图谱的全面信息，包括单核苷酸变异（SNVs）、拷贝数变异（CNVs）和表观遗传变异。将ctDNA与HCC中的传统血清蛋白生物标志物结合将为无创监测实时肿瘤进展提供新的可能。
学问题。 案例一：多组学分析揭示肝癌起始细胞免疫逃逸机制和精准治疗策略 发表期刊：Cancer Cell 影响因子：44.5 发表时间：2024 年 12 月 研究疾病：肝细胞癌（Hepatocellular Carcinoma,HCC） 样本类型：患者来源的肝癌类器官（HCC organoids），肝癌细胞系（Hep3B,Huh7,PLC/PRF/5, MHCC97 H,Hep53.4）和小鼠模型（包括自发性肝癌模型和异种移植模型） 样本数量：11 个 HCC 类器官样本，4 个肝癌细胞系样本
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HCC1187
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