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- 生长状态：
悬浮生长，多细胞聚集
- 细胞形态：
上皮样
- 器官来源：
胃
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 相关疾病：
胃癌
- 库存：
大量
- 运输方式：
冻存运输
- ATCC Number：
CRL-5973™
- 年限：
33 years
| Designations: | SNU-5 | ||
| Depositors: | J Park | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: stomach Disease: gastric carcinoma Derived from metastatic site: ascites |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1987 | ||
| Receptors: | acetylcholine, muscarinic, expressed vasoactive intestinal peptide (VIP), expressed vasoactive intestinal peptide (VIP); acetylcholine, muscarinic |
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| Oncogene: | myc +; erb B2 + | ||
| Antigen Expression: | Blood Type O; Rh + The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72. |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 8,9 D16S539: 13 D5S818: 10 D7S820: 8,12 THO1: 9 TPOX: 11 vWA: 15,16 |
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| Cytogenetic Analysis: | This is a hypotetraploid human cell line with the modal chromosome number of 87 and 88 occurring in a total of 36% of cells. Cells having higher ploidies were found at 9.6%. Eighteen or more marker chromosomes were found in most cells, including paired der(1)t(1;21) (p36.3;q11.2); der(1)t(1;?) (p32;?); 7q+'s; del(6) (q23); 12q+; and i(17q); triple der(2)t(2;?13) (p25.3;q14.3); and other single markers. There are two kinds of 7q+'s: der(7)t(7;?) (q22.3;?) and der (7)t(7;?) (q32;?). Multiple copies of DMs were also found in most cells. Normal N1 was absent. N5, N7, N10, N19 and N20 had four copies in most cells. At least one normal X chromosome was detected, but the normal Y was absent. | ||
| Age: | 33 years | ||
| Gender: | female | ||
| Ethnicity: | Asian | ||
| Comments: | SNU-5 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The patient had previously received chemotherapy including 5-fluorouracil, doxorubicin and mitomycin-C. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-5 cells were positive for VIP receptors but lacked gastrin receptors. They expressed receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The cells expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 34 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
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| References: | 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. |
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