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- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
胃
- 年限：
44 years adult
- 生长状态：
悬浮生长，多细胞聚集
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 相关疾病：
胃癌
- ATCC Number：
CRL-5971™
| Designations: | SNU-1 | ||
| Depositors: | J Park | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: stomach Disease: gastric carcinoma Derived from metastatic site: ascites |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1984 | ||
| Receptors: | vasoactive intestinal peptide (VIP), expressed | ||
| Oncogene: | myc +; erb B2 + | ||
| Antigen Expression: | Blood Type O; Rh + The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72. |
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| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 12,13 D13S317: 11,14 D16S539: 9,12 D5S818: 12,13 D7S820: 9,12 THO1: 8,9.3 TPOX: 8,10 vWA: 14 |
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| Cytogenetic Analysis: | SNU-1 is a near-diploid cell line with a modal chromosome number of 47; double minute chromosomes were found in 28% of the cells. They have a reported colony-forming efficiency of 1.9% in semisolid medium and a population doubling time of 26 hours. The cells grow as floating aggregates of round cell clusters. This is a hyperdiploid human cell line with the modal chromosome number of 47 occurring in 94% of cells. Higher ploidies occurred at 42%, which is very high. The karyotype of 47,XY,t(1;4) (q24;q33), +M1 is very uniform and stable. M1 is an F- sized small metacentric chromosome. | ||
| Age: | 44 years adult | ||
| Gender: | male | ||
| Ethnicity: | Asian | ||
| Comments: | SNU-1 was derived in 1984 by J. Park and associates from a poorly differentiated primary carcinoma of the stomach, taken prior to cytotoxic therapy. The cells are L-dopa decarboxylase (DDC) negative. SNU-1 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 26 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. |
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文献和实验都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
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