- 询价
- 2026年01月05日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 器官来源：
胃
- 年限：
33 years
- 相关疾病：
胃癌
- 库存：
大量
- ATCC Number：
CRL-5974™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
悬浮生长，多细胞聚集
| Designations: | SNU-16 | ||
| Depositors: | J Park | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: stomach Disease: gastric carcinoma Derived from metastatic site: ascites |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1987 | ||
| Receptors: | vasoactive intestinal peptide (VIP), expressed [23078 ] | ||
| Oncogene: | myc +; erb B2 + | ||
| Antigen Expression: | Blood Type A; Rh + The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72. |
||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 8,12 D16S539: 11,13 D5S818: 10,13 D7S820: 12 THO1: 6,9 TPOX: 11 vWA: 16 |
||
| Cytogenetic Analysis: | This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found. | ||
| Age: | 33 years | ||
| Gender: | female | ||
| Ethnicity: | Asian | ||
| Comments: | SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Doubling Time: | 27 hrs | ||
| References: | 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Science 子刊揭露遗传性肺癌亚型，我们离肺癌精准医疗还有多远？
Medicine 2021 年 1 月 27 号，来自美国国家癌症研究所（NCI）的 Anish Thomas 教授带领团队在 Science 子刊 Science Translational Medicine 上发表了题为 Whole-exome sequencing reveals germline-mutated small cell lung cancer subtype with favorable response to DNA repair–targeted therapies 的研究
Basic Principles for the Study of Metastasis Using Animal Models
have become generally available. For more than 20 years, the mainstays of cancer research were a handful of transplanted rodent tumors. A few of these (most notably B16F10 and Lewis lung carcinoma) were used as metastasis models, generally by injecting the cells
受体细胞株(B类)DU145 人前列腺癌细胞G-401 人肾癌WilmsGLC-82 人肺腺癌细胞GT1.1 人垂体瘤细胞（分泌促生长激素分泌激素）HCT116 人结直肠癌HCT-8 人结肠腺癌HEC-1B 人子宫内膜癌细胞Hela 人宫颈癌细胞Hep G2 人肝癌细胞Hep-2 人喉癌细胞HL-60 人白血病细胞HOS 人骨肉瘤细胞HT-1080 人纤维肉瘤HT-29 人结肠腺癌细胞HuTu-80 人十二脂肠腺癌JEG-3 人绒癌细胞JEG-3/VP16 人绒癌
技术资料暂无技术资料 索取技术资料
