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- 2026年01月03日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
- 年限：
65 years
- 生长状态：
贴壁生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
子宫
- 相关疾病：
肿瘤
- ATCC Number：
CRL-1671™
- 组织来源：
endometrium
- 运输方式：
冻存运输
- 细胞形态：
上皮样
| Designations: | RL95-2 | ||
| Depositors: | DL Way | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: uterus Tissue: endometrium Disease: carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | estrogen receptor, positive | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 8,12 D16S539: 11,13 D5S818: 10,11 D7S820: 10 THO1: 9,9.3 TPOX: 8 vWA: 16,20 |
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| Age: | 65 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 |
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| References: | 2151: Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371 22666: Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137 29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 32565: Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448 |
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文献和实验RNA Markers的使用方法(适用于RNA Marker RL1,000、RL6,000、RL10,000）
RNA Marker ( RL1,000; RL6,000; RL10,000)可以进行一般琼脂糖凝胶电泳和变性琼脂糖凝胶电泳。以RL10,000为例，具体使用方法如下：普通琼脂糖凝胶电泳时1.按下列组份配置RNA Marker样品。2.均匀混合后65℃加热10分钟，迅速冷却至室温（最好用PCR仪)。3.使用高质量的琼脂糖，用1×TAE Buffer制备3%凝胶*，制胶时凝胶中请加入溴乙锭(最终浓度：1 μg/ml)。4.将上述操作2配制的Marker样品加样后，在1×TAE Buffer中
RNAMarkers的使用方法(适用于RNAMarkerRL1,000、RL6,000、RL1
RNA Marker ( RL1,000; RL6,000; RL10,000) 可以进行一般琼脂糖凝胶电泳和变性琼脂糖凝胶电泳。 以RL10,000为例，具体使用方法如下： 普通琼脂糖凝胶电泳时 1.按下列组份配置RNA Marker样品。 2.均匀混合后65℃加热10分钟，迅速冷却至室温（最好用PCR仪)。 3.使用高质量的琼脂糖，用1×TAE Buffer制备3%凝胶*，制胶时凝胶中请加入溴乙锭(最终浓度：1 μg
RNA Markers的使用方法(适用于RNA Marker RL1,000、RL6,000、RL10,000
RNA Marker ( RL1,000; RL6,000; RL10,000) 可以进行一般琼脂糖凝胶电泳和变性琼脂糖凝胶电泳。 以RL10,000为例，具体使用方法如下： 普通琼脂糖凝胶电泳时 1.按下列组份配置RNA Marker样品。 2.均匀混合后65℃加热10分钟，迅速冷却至室温（最好用PCR仪)。 3.使用高质量的琼脂糖，用1×TAE Buffer制备3%凝胶
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