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- 详细信息
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞类型：
B淋巴细胞
- 组织来源：
upper maxilla
- 相关疾病：
淋巴瘤
- ATCC Number：
HTB-60™
- 细胞形态：
淋巴样
- 年限：
9 years
| Designations: | EB1 | ||
| Depositors: | G Trempe, LJ Old | ||
| Biosafety Level: | 2 [Cells Contain HERPESVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Tissue: upper maxilla Disease: Burkitt's lymphoma Cell Type: B lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isoenzymes: | ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 |
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| Age: | 9 years | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| Comments: | The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma. Viral particles (Epstein-Barr virus) were noted through electron microscopic examination. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Twice per week Allow cells to settle, remove most of the supernatant medium, dilute with fresh medium to 4 X 10 exp5 viable cell/ml. Subculture at time of fluid renewal. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852 22169: Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790 22722: . . Br. J. Cancer 19: 108-115, 1965. 23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014 26323: . . J. Exp. Med. 121: 761-770, 1965. 26324: Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357 26325: Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453 26326: Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. PubMed: 6818551 |
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文献和实验收集在AnchorChip，并进行作质谱分析，用自动化Mascot进行数据搜索。结果 大部分视网膜蛋白可以在Tris缓冲液（EB1）及进一步的含尿素及CHAPS（分别含量为70%和28%）的缓冲液（EB2）中溶解。只有很少视网膜蛋白可溶解在SDS缓冲液（EB4）中。在2-D电泳中分离出的蛋白质特性与在EB1及EB2中分离出的蛋白质特性相似。很少部分的蛋白质只能经EB2萃取后经2-D电泳分离到。在机器自动化蛋白质分析鉴定中，总的成功检出率为67.8%。特别是那些有较好MS谱图的蛋白分子有着更高的检出率（>90%）。已鉴定
Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy
. Here, we describe the purification of EB1 and CLIP-170, the total internal reflection fluorescence microscopy assay to observe dynamic end tracking in vitro, and the quantitative analysis of fluorescent +TIP comet shape and of single +TIP molecule turnover at growing microtubule
-MULV反转录酶 1 μL 置于 42℃水浴，1h。 ③ 70℃保温5min（使酶失活）。 ④ 于-20℃保存备用。 PCR 扩增目的基因 以反转录产物为模板，克隆引物B1，B2为引物，利用rTaq酶进行扩增目的基因，用于后续连入克隆载体的操作，然后再以表达引物EB1，EB2为引物，利用Pfu DNA聚合酶进行扩增目的基因，用于后续连入表达载体的操作。 94 ℃ 3 min 94 ℃ 45Sec
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