- 询价
- 2026年01月17日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞类型：
其他细胞类型
- 相关疾病：
其他疾病
- ATCC Number：
TIB-161™
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 年限：
53 years adult
- 生长状态：
悬浮生长
- 库存：
大量
| Designations: | HuT 78 | ||
| Depositors: | AF Gazdar | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
||
| Source: | Disease: Sezary Syndrome Cell Type: cutaneous T lymphocyte; |
||
| Cellular Products: | interleukin 2 [1140 ] tumor necrosis factor alpha (TNF alpha) [23420 ] |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Receptors: | interleukin 2 (IL-2), expressed [1140 ] | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | CD4; Homo sapiens [22610 ] | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11,12 D5S818: 11,12 D7S820: 8,11 THO1: 8,9 TPOX: 8,9 vWA: 14,15 |
||
| Age: | 53 years adult | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | H9 (ATCC HTB-176 ) is a clonal derivative of HuT 78 [Pubmed: 2567177]. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp4 and 8 X 10 exp5 viable cells/ml. maintain cell density at less than 1 X 10 exp6 cells/ml. Medium Renewal: Two to three times weekly |
||
| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Doubling Time: | about 65 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 derivative:ATCC HTB-176 |
||
| References: | 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346 22484: Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177 22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013 23228: Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674 23420: O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285 32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991 32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 32796: Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
CDw78 分子 CDw78 常用单克隆抗体或代号： Leu21,Ba抗原 主要表达细胞： B,Macsub [B] 分子质量(kDa)和结构： p67? 功 能： B细胞活化的辅助蛋白 CDw78 Deleted at 7th HLDA Workshop
Polynucleotide Kinase (EC 2.7.1.78)
The enzyme polynucleotide kinase (PNK, or ATP:5′-dephospho-polynucleotide 5′-phosphatase) catalyzes the transfer of a γ-phosphate group from a 5′-nucleoside triphosphate moiety to a free 5′-hydroxyl of a polynucleotide such as DNA or RNA, to form
1．硬度碎片抽屉 2．硬度微调夹头 3．硬度盒盖 4．电源指示灯 5．电热保温指示灯 6．硬度指示读数表 7．脆碎盒盖 8．崩解升降导杆 9．升降导杆接杆 10．崩解支杆 11．释放水平调节螺钉 12．崩解吊篮夹头 13．释放支杆 14．释放转动轮和皮带 15．释放转篮杆 16．热敏电阻 17．释放转篮 18．温度探头插头座 19．崩解吊篮 20．1000m1烧杯 21．水箱 22．崩解、释放选择开关 23．硬度、脆碎选择
技术资料暂无技术资料 索取技术资料
HuT 78
询价
