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- 技术资料
- 相关疾病：
肝癌
- 器官来源：
肝
- ATCC Number：
CRL-10741™
- 年限：
15 years adolescent
- 生长状态：
贴壁生长
- 库存：
大量
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | C3A [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)] | ||
| Depositors: | Baylor College of Medicine | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Disease: hepatocellular carcinoma |
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| Cellular Products: | alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein) |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | No | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 9,13 D16S539: 12,13 D5S818: 11,13 D7S820: 10 THO1: 9 TPOX: 8,9 vWA: 17 |
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| Age: | 15 years adolescent | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium. As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion. Gluconeogenesis activity is strongly oxgen dependent. The cells have nitrogen metabolizing activity comparable to perfused rat livers. There is no evidence of a Hepatitis B virus genome in this cell line. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
|
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 parental cell line:ATCC HB-8065 |
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| References: | 22052: Kelly JH. Permanent human hepatocyte cell line and its use in a liver assist device (LAD). US Patent 5,290,684 dated Mar 1 1994 | ||
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文献和实验相关实验
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠补体C3a (C3a)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 C3a 单抗包被于酶标板上，标准品和样品中的 C3a与单抗结合，加入生物素化的抗小鼠 C3a
小鼠 补体3a（C3a） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 补体3a(C3a) 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 补体3a （ C3a ） 水平。用纯化的 小鼠 C3a 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 补体3a （ C3a ）， 再与HRP 标记的 C3a 抗体
人补体C3a (C3a)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 C3a 单抗包被于酶标板上，标准品和样品中的 C3a与单抗结合，加入生物素化的抗人 C3a ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入
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C3A
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