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- 询价记录
- 文献和实验
- 技术资料
- 运输方式:
冻存运输
- 物种来源:
人
- 是否是肿瘤细胞:
1
- 器官来源:
乳房
- 年限:
43 years
- 细胞形态:
其他
- 相关疾病:
腺癌
- ATCC Number:
HTB-130™
- 库存:
大量
- 生长状态:
贴壁生长
| Designations: | MDA-MB-436 | ||
| Depositors: | R Cailleau | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | pleomorphic with multinucleated component cells |
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| Source: | Organ: mammary gland; breast Disease: adenocarcinoma Derived from metastatic site: pleural effusion |
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| Cellular Products: | tubulin; actin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | No | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 9,11 D5S818: 13 D7S820: 10 THO1: 9.3 TPOX: 8 vWA: 14,20 |
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| Cytogenetic Analysis: | modal number = 45 | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
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| Age: | 43 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining. | ||
| Propagation: | ATCC complete growth medium: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10% Temperature: 37.0°C Atmosphere: air, 100% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 2 to 3 times per week Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 |
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文献和实验Bcap-37和MDA-MB-435细胞都是人乳腺癌细胞,培养液用DMEM或1640均可,小牛血清10%就足够,不过感觉435细胞用DMEM长得更旺一些。两种细胞都很好养,Bcap-37可称为是最漂亮的乳腺癌细胞,一个个成排列均匀的瓜子状,形态规则,长得也快,一般1:2传代后2-3天即可长满。435长的较慢,需4-5天。传代常规用0.25%的胰酶消化后用含血清的培养基终止消化,视消化程度而决定是否需要离心,传入新瓶加入培养液即可。需要注意的是传代时的密度,特别是435密度低时很难生长。一般生长
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的新陈代谢来补充这种能量,因此 Goun 想要研究 NR 在癌症发展和扩散中的作用。 通过使用 BiNR 探针,研究人员对四种人乳腺癌细胞系(MDA-MB-231-luc、MDA-MB-468-luc、MCF-7-luc 和 BT-20-luc)的烟酰胺核糖摄取进行了量化,并发现一种高侵略性的三阴乳腺癌细胞系对于烟酰胺核糖的摄取率最高。 接下来,他们 MDA-MB-231 异种移植肿瘤利用小鼠模型,探索烟酰胺核糖是否会增加癌症发病和转移。令人惊讶的是,研究结果显示,烟酰胺核糖的补充会导致小鼠
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