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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 库存：
大量
- 细胞形态：
上皮样
- 年限：
56 years
- 生长状态：
贴壁生长
- 器官来源：
舌头
- 相关疾病：
鳞状细胞癌
- ATCC Number：
CRL-2095™
- 物种来源：
人
- 是否是肿瘤细胞：
1
| Designations: | CAL 27 | ||
| Depositors: | C Cardona | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: tongue Disease: squamous cell carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1982 | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 10,11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 14,17 |
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| Cytogenetic Analysis: | aneuploid; modal number = 43 | ||
| Age: | 56 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue. CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm. Immunocytochemical studies show strong positive staining with anti keratin antibodies. The cells do not grow well in semi-solid medium. Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate). CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D). A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Doubling Time: | 35 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269 | ||
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文献和实验The term ribonuclease (RNase) is an imprecise term and is used to cover both enzymes that cause exonucleolytic cleavage and endonucleolytic cleavage of RNA. Exonucleases may cleave the RNA in 3′-5′ direction or vice versa
Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
近期，来自军事医学研究院生物工程研究所的魏从文教授团队在 Molecular cancer 杂志发表了题为「GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis」的科研论文，IF = 27.7。 研究背景： 结直肠癌（Colorectal cancer，CRC）是全球第三大常见恶性肿瘤，也是导致癌症相关死亡的第二大原因。CRC 的进展是通过腺瘤到癌的序列，大约
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