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- 2025年09月04日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 相关疾病：
黑色素瘤
- ATCC Number：
CRL-9446™
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 器官来源：
皮肤
- 库存：
大量
| Designations: | CHL-1 | ||
| Depositors: | Berlex Laboratories, Inc. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: skin Disease: melanoma |
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| Cellular Products: | human tissue plasminogen activator (t-PA) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host Transfection host derived from RPMI 7932 that can grow to a higher density, namely 5 x 10(7) cells/ml. [41429 ] |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 8,11 D16S539: 13 D5S818: 11,12 D7S820: 11,13 THO1: 9,9.3 TPOX: 8 vWA: 17 |
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| Comments: | This is a derivative of the RPMI 7932 human melanoma cell line (Bowes). It produces about 0.1 mU of t-PA per cell per day. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Add fresh medium at the time of subculture |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 41429: Cashion L, et al. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991 | ||
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文献和实验Isolation of Photosystem II Reaction Center Complexes From Plants
Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt b 559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl
Analysis of Intermediates and End Products of the Chlorophyll Biosynthetic Pathway
Since the 1963 seminal review of Smith and French (62 ), our understanding of the chlorophyll (chl) biosynthetic pathway has changed dramatically. Several factors have contributed to this phenomenon, among which are: (i) development of systems
Analysis and Reconstitution of Chlorophyll-Proteins
It is hard to believe that only some 30 years ago, it was a matter of debate whether chlorophyll (Chl) and other photosynthesis pigments are protein-bound or just dissolved in plant membranes. Philip Thornber, who vividly described this debate
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