- 询价
- 2025年09月04日
14 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 器官来源：
结肠
- 生长状态：
混合型生长
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 相关疾病：
大肠癌
- ATCC Number：
CCL-222™
- 库存：
大量
- 年限：
Dukes' type D
| Designations: | COLO 205 | ||
| Depositors: | GE Moore | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed, adherent and suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: colon Tumor Stage: Dukes' type D Disease: colorectal adenocarcinoma Derived from metastatic site: ascites |
||
| Cellular Products: | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15 |
||
| Cytogenetic Analysis: | The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic. | ||
| Isoenzymes: | ES-D, 1-2 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1-2 |
||
| Age: | 70 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. The cells are CSAp negative (CSAp-). The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224 ). The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Protocol: Shake flask, transfer floating cells inot a new flask. Cells remaining attached may be removed using a standard trypsinization protocol. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 derived from same individual:ATCC CCL-224 |
||
| References: | 1046: Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251 22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 41500: Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
COLO 205
询价
