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- 询价记录
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- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
结肠
- 相关疾病：
大肠癌
- ATCC Number：
CCL-220™
- 运输方式：
冻存运输
- 生长状态：
混合型生长
- 细胞形态：
其他
- 年限：
Dukes' type C
- 库存：
大量
| Designations: | COLO 320DM | ||
| Depositors: | GE Moore | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed, adherent and suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | rounded and refractile |
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| Source: | Organ: colon Tumor Stage: Dukes' type C Disease: colorectal adenocarcinoma |
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| Cellular Products: | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11,12 D5S818: 12 D7S820: 9,12 THO1: 9 TPOX: 8,9 vWA: 15,18 |
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| Cytogenetic Analysis: | modal number = 24; range = 49 to 61. The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined. |
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| Isoenzymes: | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 |
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| Age: | 55 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line is negative for CEA, CSAp and colon antigen 3. The cells are weakly positive for keratins and vimentin. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: Liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117 22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 |
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