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- 库存：
大量
- 相关疾病：
大肠癌
- ATCC Number：
CCL-220.1™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
结肠
- 运输方式：
冻存运输
- 生长状态：
贴壁，多细胞聚集
- 年限：
Dukes' type C
- 细胞形态：
其他
| Designations: | COLO 320HSR [COLO 320 HSR] | ||
| Depositors: | GE Moore | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | loosely adherent, multicell aggregates | ||
| Organism: | Homo sapiens | ||
| Morphology: | cells are rounded and refractile |
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| Source: | Organ: colon Tumor Stage: Dukes' type C Disease: colorectal adenocarcinoma |
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| Cellular Products: | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11,12 D5S818: 12 D7S820: 9,12 THO1: 8,9 TPOX: 8,9 vWA: 15,18 |
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| Cytogenetic Analysis: | marker chromosomes with homogeneously staining regions (HSR) were observed and the double minute (DM) chromosomes appeared in much lower frequency than the parental line COLO 320DM | ||
| Isoenzymes: | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 |
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| Age: | 55 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | CEA, negative. CSAp, negative. Colon antigen 3, positive. The cells are weakly positive for keratins and vimentin. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a 0.25% trypsin, 0.53 mM EDTA solution. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 3 to 4 days |
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| Preservation: | Freeze medium: FreezeMedium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-220 |
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| References: | 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117 22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 |
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文献和实验以下操作按照天根产品 DP320 新型植物基因组 DNA 提取试剂盒的说明书进行，所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品，样本量不同可能现象与本文有所差异。实验准备：1. 植物叶片2. 研钵 液氮3. 移液器及配套无菌枪头（10 μl ，200 μl ，1ml），1.5 ml离心管4. 无水乙醇5. 涡旋振荡器，金属浴/水浴，台式离心机实验准备-试剂盒准备：使用前先在漂洗液PW和LP3中加入无水乙醇，加入体积请参照瓶上的标签。本文版权归天根生化科技（北京）有限
位数到达所要的数值时，按一下（读数），十位数和个位数均保持不变，小数点后十分位开始在“0”和“5”之间变化。当到达需要数值时按（读数），温度值将固定，且小数点停止闪烁，此时温度值已被读入pH计。完成温度输入后，按（模式）回到pH或mV方式。 注意：在温度输入后，但在未退出温度方式前想改变温度设定值，只需按一下（读数）使小数点闪烁，然后按（校准），照上述步骤重新输入温度值。在温度输入过程中，若想重新输入温度，按（校准），然后按上述步骤重新输入温度值。 320-S pH计在关机后将仍保留此温度值
/LGCAdvancedCatalogueSearch/tabid/961/Default.aspx?CollectionID=106 ATCC® Number Description Designation CCL-218 Homo sapiens (human) WiDr CCL-220 Homo sapiens (human) COLO 320DM CCL-220.1 Homo sapiens (human) COLO 320HSR [COLO 320 HSR] CCL-221 Homo
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