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- 细胞形态：
上皮样
- 相关疾病：
其他疾病
- 运输方式：
冻存运输
- ATCC Number：
CRL-1720™
- 器官来源：
睾丸
- 年限：
embryo
- 生长状态：
贴壁生长
- 库存：
大量
- 品系：
129
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | F9 | ||
| Depositors: | S Strickland | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial |
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| Source: | Organ: testis Strain: 129 Disease: embryonal carcinoma; testicular teratoma |
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| Cellular Products: | plasminogen activator; laminin; type IV collagen | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Age: | embryo | ||
| Comments: | F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP). Differentiating cells synthesize plasminogen activator, laminin and type IV collagen. cAMP is active only on cells that have been treated with retinoic acid. The cells maintain three copies of the beta 1 integrin gene. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: NOTE: culture vessels must be coated with 0.1% gelatin prior to use.To do so, cover the surface of the vessel with 0.1% gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperature.
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 1160: Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719 1161: Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238 23426: Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677 26151: Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215 32547: Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 |
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。 洁净室末端高效过滤器的使用寿命应为5～15年，影响使用寿命的最主要因素是预过滤器的优劣。 当使用“G～F～H～U”效率规格分类时，可方便地估计所需各级过滤器的效率。在G2～H12中，每隔2～4档设置一级过滤器。例如：G4→F7→H10，其中，末端H10（亚高效）过滤器决定送风的洁净水平，F7保护H10，G4保护F7。 洁净室末端高效（HEPA）过滤器前要有效率规格不低于F8的过滤器来保护；超高效（ULPA）过滤器前可选用F9～H11的过滤器。中央空调本身应有效率规格不低于F5的过滤
基因打靶资料 http://www.dxy.cn/bbs/post/view?bid=41&id=700550&sty=3&keywords=%BB%F9%D2%F2+%B4%F2%B0%D0 http://www.dxy.cn/bbs/thread/1274625 http://www.dxy.cn/bbs/post/view?bid=64&id=544760&sty=3&keywords=%BB%F9%D2%F2+%B4%F2%B0%D0 http://www.dxy.cn/bbs
Crystallization of Kinesin Family Motor Proteins
Kar3+N11 (WT)PDB: 1F9TP21a=43.6 Åb=78.8 Åc=47.2 Åß=105.0°1.5 ÅMolecular replacement Kar3 N650KPDB: 1F9UP21a=43.6 Åb=78.0 Åc=47.3 Åß=105.1°1.7 ÅMolecular replacement Kar3 R598APDB: 1F9VP21a=43.9 Åb=77.4 Åc=47.7 Åß=105.9°1.3 ÅMolecular replacement Kar3 E
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