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- 技术资料
- 库存：
大量
- 生长状态：
悬浮生长
- 免疫类型：
IgM; kappa light chain
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- ATCC Number：
HB-8051™
| Designations: | F5-A-1/22.8.13 | ||
| Depositors: | Research Corporation | ||
| Isotype: | IgM; kappa light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human prostate antigen (PA) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with purified PA. Spleen cells were fused with P3X63Ag8 myeloma cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | fetal bovine serum, 90%; DMSO, 10% | ||
| References: | 3874: Wang MC, et al. Purified human prostate antigen. US Patent 4,446,122 dated May 1 1984 58335: Wang MC, et al. Purification of a human prostate specific antigen. Invest. Urol. 17: 159-163, 1979. PubMed: 89106 |
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文献和实验a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
on ice for 20 min. 13. Centrifuge at 1,000 X g for 5 min to collect the precipitate. 14. Discard the supernatant and dry the inside of the tube with a paper towel (do not dry the pellet). 15. Dissolve DNA in 900 μl of TE Buffer by gentle
)、F4系列(F4、F4S、F4E)、F-601(N6006)、F90(N90)、F90X(N90X)、F70(N70)、F50(N50)及最终的F5，除了F5、F4、F90X、F70、F50还在生产外，其余均已停产。 F-501是最早推出的，实质上是F-301加上AF系统而成，所以F-301的毛病它都有，此机早已过时，此处免谈。但有一点不可不提，它的机身是由两块铸铝组成的，其结实程度要比以后的FXXX和FXX们强多了。 F-401系列
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