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- 库存：
大量
- 运输方式：
冻存运输
- 器官来源：
食道
- ATCC Number：
CRL-2692™
- 细胞形态：
上皮样
- 年限：
25 years adult
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 细胞类型：
其他细胞类型
| Designations: | Het-1A | ||
| Depositors: | CC Harris | ||
| Biosafety Level: | 2 [cells contain SV40 viral sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: esophagus Cell Type: epithelialSV40 large T antigen transfected |
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| Cellular Products: | cytokeratin [56132 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The cells are distributed for research purposes only. The National Cancer Institute (NCI) releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Neither the cell line nor products derived from it may be sold or used for commercial purposes. 2.) Any commercial requests or proposed commercial use of these cells must first be negotiated with the Office of Technology Transfer, NIH, 6011 Executive Blvd., Room 325, Rockville, MD 20852; Telephone (301) 496-7057; FAX (301) 402-0220 | ||
| Isolation: | Isolation date: 1986 | ||
| Tumorigenic: | No | ||
| Antigen Expression: | SV40 T antigen [56132 ] [57804 ] | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 9,11 D5S818: 11,12 D7S820: 9 THO1: 7 TPOX: 11 vWA: 16 |
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| Cytogenetic Analysis: | hypodiploid (34-40 chromosomes) [56132 ] | ||
| Age: | 25 years adult | ||
| Gender: | male | ||
| Ethnicity: | Black | ||
| Comments: | The HET-1A cell line was derived in 1986 from human esophageal autopsy tissue by transfection with plasmid pRSV-T consisting of the RSV-LTR promoter and the sequence encoding the simian virus 40 large T-antigen. [56132 ] The line has undergone more than 250 population doublings. [56132 ] Growth factor studies have shown that HET-1A is stimulated by calcium and inhibited by fetal bovine serum, transforming growth factor-beta 1, and transforming growth factor-beta 2. [56132 ] Fumonisin B1 produces growth inhibition and increased apoptosis in HET-1A cells. [57803 ] [57804 ] The synthetic retinoid CD437 (6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (AHPN/CD437)0 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway. [57805 ] The cell line may be useful for investigating the action of putative esophageal carcinogens. [56132 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit. Note: Do not filter complete medium. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove the spent medium, and add 0.05% trypsin -0.53 mM EDTA solution containing 0.5% polyvinylpyrrolidone (PVP) and allow the culture to sit at room temperature until the cells begin to detach (usually 5 to 10 minutes). Neutralize the trypsin with 0.1% soybean trypsin inhibitor. Resuspend the cells in fresh medium, aspirate and dispense into coated flasks. The flasks used should be precoated with with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen type I and 0.01 mg/ml bovine serum albumin dissolved in culture medium. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Leibovitz's L-15 medium with 2 mM L-glutamine and 10mM HEPES supplemented with 1% PVP, 10% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
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| References: | 56132: Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 57802: Milo GE, et al. Conversion of premalignant human cells to tumorigenic cells by methylmethane sulfonate and methylnitronitrosoguanidine. Cell Biol. Toxicol. 8: 193-205, 1992. PubMed: 1337307 57803: Tolleson WH, et al. Apoptotic and anti-proliferative effects of fumonisin B1 in human keratinocytes, fibroblasts, esophageal epithelial cells and hepatoma cells. Carcinogenesis 17: 239-249, 1996. PubMed: 8625445 57804: Tolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392: 237-250, 1996. PubMed: 8850621 57805: Wan X, et al. Synthetic retinoid CD437 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway. Anticancer Res. 21: 2657-2663, 2001. PubMed: 11724335 |
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