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- ATCC Number：
TIB-149™
- 运输方式：
冻存运输
- 库存：
大量
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | Ig(5a)7.2 (formerly 10-4-22) | ||
| Depositors: | LA Herzenberg | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against the Ig-5a allotype on mouse IgD | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with antigen - antibody complexes (anti Bordetella pertussis with B. pertussis). Spleen cells were fused with NS-1 myeloma cells. This line is identical to ATCC TIB-97. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
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| References: | 940: Oi VT, Herzenberg LA. Localization of murine Ig-1b and Ig-1a (IgG2a) allotypic determinants detected with monoclonal antibodies. Mol. Immunol. 16: 1005-1017, 1979. PubMed: 94425 2126: Oi VT, et al. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr. Top. Microbiol. Immunol. 81: 115-129, 1978. PubMed: 567555 |
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文献和实验10.1 30.0 20.0 10.2 33
【操作步骤】6.2 分文章作者，亲手教你如何轻松搞定生信文章
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Ig分子由4条肽链组成，2条长链称为重链（heavychain，H），由大约440个氨基酸残基组成，分子量约50～70kD；2条短链称为轻链（lightchain，L），由大约220个氨基酸组成，分子量约22.5kD.4条肽链通过链间二硫键（-S·S-）连在一起。其结构模式见图2-1. Ig分子肽链的N端，在L链1/2和H链1/4处（约在110位前）氨基酸的种类和顺序各不相同，称为可变区（variableregion，V区）；肽链C端其余部分的氨基酸，在种类和顺序上彼此间差别不大，称为
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