- 询价
- 2026年02月09日
企业认证
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 细胞形态：
上皮样
- ATCC Number：
CCL-13™
- 运输方式：
冻存运输
- 器官来源：
其他
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
| ##Caption## | |||
| Designations: | HeLa [Chang Liver] | ||
| Depositors: | RS Chang | ||
| Biosafety Level: | 2 [Cells contain human papilloma virus (HPV-18) ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: HeLa contaminant | ||
| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
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| Isoenzymes: | G6PD, A | ||
| HeLa Markers: | Y | ||
| Comments: | This line was originally thought to be derived from normal liver tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining. NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination |
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| Propagation: | ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow flask to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Complete growth medium, 95%; DMSO, 5% | ||
| References: | 22213: Chang RS. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440-443, 1954. PubMed: 13237268 26122: Murphy WH, Landau BJ. Clonal variation and interaction of cells with viruses. Natl. Cancer Inst. Monogr. 7: 249-271, 1962. PubMed: 14477443 32352: Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004 |
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文献和实验identical to HeLa. These include Hep 2,WISH, INT 407, Chang liver and Giradi heart. To minimize the risk of working with contaminated cell lines it is advisable to obtain cells from a recognized source such as a culture collection that will have confirmed
identical to HeLa. These include Hep 2,WISH, INT 407, Chang liver and Giradi heart. To minimize the risk of working with contaminated cell lines it is advisable to obtain cells from a recognized source such as a culture collection that will have confirmed
) and ERK5 have been found to be expressed in mammals (Gutkind, 2000; Chang and Karin, 2001).This study is conducted to investigate the effects of clivorine on the growth of human normal liver L-02 cells and the potential effects on two major MAPKs: p38
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