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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 细胞类型：
成纤维细胞
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- 相关疾病：
正常
- 库存：
大量
- ATCC Number：
CCL-137™
- 器官来源：
肺
- 年限：
fetus
| Designations: | HEL 299 | ||
| Depositors: | RG Brackett | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: lung Disease: normal Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | acetylcholine, muscarinic m2 | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 7,10 D13S317: 11,12 D16S539: 10,11 D5S818: 11,13 D7S820: 8,11 THO1: 7 TPOX: 8,12 vWA: 16 |
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| Cytogenetic Analysis: | normal human male; diploid; stable | ||
| Isoenzymes: | G6PD, A | ||
| Age: | fetus | ||
| Gender: | male | ||
| Ethnicity: | Black | ||
| Comments: | The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 18 population doublings before the onset of senescence. M2 muscarinic receptor expression is downregulated following protein kinase C stimulation. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 1071: Peterson WD Jr., et al. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. PubMed: 5668122 23419: Rousell J, et al. Transcriptional down-regulation of m2 muscarinic receptor gene expression in human embryonic lung (HEL 299) cells by protein kinase C. J. Biol. Chem. 270: 7213-7218, 1995. PubMed: 7706260 29070: Shimamoto T, et al. Inhibition of DLX-7 homeobox gene causes decreased expression of GATA-1 and c-myc genes and apoptosis. Proc. Natl. Acad. Sci. USA 94: 3245-3249, 1997. PubMed: 9096378 32249: Campbell S, et al. Evidence for ethnic tropism of Helicobacter pylori. Infect. Immun. 65: 3708-3712, 1997. PubMed: 9284141 32718: Muralidhar S, et al. Human cytomegalovirus mtrII oncoprotein binds to p53 and down-regulates p53-activated transcription. J. Virol. 70: 8691-8700, 1996. PubMed: 8970996 |
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HEL 299
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