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- 文献和实验
- 技术资料
- 细胞形态：
神经元
- 生长状态：
贴壁生长
- 年限：
7 years
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 器官来源：
大脑
- ATCC Number：
CRL-10742™
- 运输方式：
冻存运输
- 库存：
大量
| Designations: | HCN-2 | ||
| Depositors: | Johns Hopkins University | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | neuronal |
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| Source: | Organ: brain Cell Type: cortical neuron; |
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| Cellular Products: | tubulin; neurofilament protein; somatostatin; cholecystokinin-8 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | The cells are negative for glial fibrillary acidic protein (GFAP) and myelin basis protein (MBP). The cells stain positively for a number of neuronal markers including neurofilament protein, neuron specific enolase (NSE). The HCN-2 cell line was derived from cortical tissue removed from a patient undergoing hemispherectomy for intractable seizures associated with Rasmussen's encephalitis. They are also positive for tubulin, vimentin, somatostatin (SST), glutamate, gamma aminobutyric acid (GABA), cholecystokinin - 8 (CCK-8) and vasoactive intestinal peptide (VIP). � HCN-2 cells can be induced to differentiate when cultured with a mixture of nerve growth factor (NGF), dibutyryl cyclic adenosine monophosphate (cAMP) and 1-isobutyl-3-methylxanthine (IBMX). |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,15 D13S317: 11,12 D16S539: 8,11 D5S818: 13 D7S820: 9,11 THO1: 6,9.3 TPOX: 8 vWA: 14,20 |
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| Age: | 7 years | ||
| Gender: | female | ||
| Comments: | HCN-2 cells can be induced to differentiate when cultured with a mixture of nerve growth factor (NGF), dibutyryl cyclic adenosine monophosphate (cAMP) and 1-isobutyl-3-methylxanthine (IBMX). Differentiation is accompanied by mature morphology and slowing of growth (doubling time greater than 120 hours). The growth rate of HCN-2 cells is stimulated by treatment with phorbol esters. A similar line (HCN-1A) is available as ATCC CRL-10442. The HCN-2 cell line was derived from cortical tissue removed from a patient undergoing hemispherectomy for intractable seizures associated with Rasmussen's encephalitis. The cells stain positively for a number of neuronal markers including neurofilament protein, neuron specific enolase (NSE). They are also positive for tubulin, vimentin, somatostatin (SST), glutamate, gamma aminobutyric acid (GABA), cholecystokinin - 8 (CCK-8) and vasoactive intestinal peptide (VIP). The cells are negative for glial fibrillary acidic protein (GFAP) and myelin basis protein (MBP). CRL-10742 has been shown to senesce at approximately passage 21. Current distribution stocks are prepared with only 4-5 Population Doublings (PDLs) remaining under recommended culture conditions after cryopreservation. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: NOTE: This cell line is extremely slow growing. May not be able to subculture for 12 to 14 days after recovery. Cultures do not reach 100% confluence (about 80-90%). A change in the fetal bovine serum may help increase the growth rate. Subculture at no higher than a 1:3 ratio every 10 to 12 days when cultures are about 90% confluent.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 22022: Ronnett GV, et al. Human neuronal cell line. US Patent 5,196,315 dated Mar 23 1993 | ||
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HCN-2
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