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- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 器官来源：
腋窝淋巴结
- 运输方式：
冻存运输
- 年限：
adult
- ATCC Number：
CRL-2161™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 组织来源：
vascular endothelium
- 库存：
大量
- 品系：
C3H/HeJ
- 细胞类型：
SV40转化细胞
| Designations: | SVEC4-10EHR1 | ||
| Depositors: | KA O'Connell | ||
| Biosafety Level: | 2 [Cells contain polyomavirus DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial |
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| Source: | Organ: axillary lymph node Strain: C3H/HeJ Tissue: vascular endothelium Cell Type: endothelialSV40 transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | H-2 K; VCAM | ||
| Age: | adult | ||
| Gender: | male | ||
| Comments: | SVEC4-10EHR1 is an endothelial cell line derived from the same solid tumor as SVEC4-10EE2 (see ATCC CRL-2167 ) in a nude mouse injected with SVEC4-10 (see ATCC CRL-2181 ). SVEC4-10EHR1 was cloned from cells from the SVEC4-10 induced tumor in 1990 by limiting dilution using medium conditioned by SVEC4-10EE2. The cell line is distinguished by its spindle cell morphology both in vitro and in vivo in contrast to SVEC4-10EE2. SVEC4-10EHR1 expresses the cell surface major histocompatibility complex class I antigen (H-2 k) of the parental cell line, and express the vascular cell adhesion molecule (VCAM). The cells stain positively for SV40 T antigen. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Twice per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 23187: O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 |
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文献和实验相关实验
Jinno K, Nakamura H. Chromatographia. 1994,39:285-93. [5] Svec F, Frechet J M, J. Chromatogr. A 1995,702:89-95. [6] Danielson N D, Wangsa J, Shamsi S A. J. Liq. Chromatogr.1995,18:2579-92. [7] Smigol, V, Svec F, Frechet J M. J. Anal. Chem. 1994,66
:285-93. [5] Svec F, Frechet J M, J. Chromatogr. A 1995,702:89-95. [6] Danielson N D, Wangsa J, Shamsi S A. J. Liq. Chromatogr.1995,18:2579-92. [7] Smigol, V, Svec F, Frechet J M. J. Anal. Chem. 1994,66:4308-15. [8] Sander L C, Wise, S. A.LC-GC
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SVEC4-10EHR1
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