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Ha Fe

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  • 2025年09月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 器官来源

      皮肤

    • 细胞形态

      成纤维样

    • 年限

      7 days

    • 相关疾病

      其他疾病

    • ATCC Number

      CRL-1396™

    • 物种来源

    • 是否是肿瘤细胞

      0

    • 运输方式

      冻存运输

    • 生长状态

      贴壁生长

    Designations: Ha Fe
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: fibroblast

    Source: Organ: skin
    Disease: cutis laxa
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    DNA Profile (STR): Amelogenin: X,Y
    CSF1PO: 10,12
    D13S317: 8,11
    D16S539: 10,11
    D5S818: 11
    D7S820: 10
    THO1: 6,9
    TPOX: 8,11
    vWA: 17,19
    Age: 7 days
    Gender: male
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37�C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: 2 to 3 times per week
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2020

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    图标文献和实验
    相关实验
    • Ig Fe受体

      佚名     Ig分为IgM、IgD、IgA、IgD和IgE五类。各类Ig的不同功能主要与其结构有关。机体内许多细胞表面具有不同类IgFe的受体,通过Fe受体与IgFe的结合,参与Ig介导的生理功能或病理损伤过程。目前已鉴定明确的Fe受体有FeγR、FcαR和FcεR. (一)FeγR 1.FeγR的结构和分布 FeγR可分为FeγRⅠ、FeγRⅡ

    • Anti-HA Immunocytology

        The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein

    • Anti-HA Immunocytology

        Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots

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