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- ATCC Number：
HB-178™
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | 4D12 | ||
| Depositors: | BF Haynes | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against HLA B5 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with the HUT 78 Sezary cutaneous T cell line. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody reacts with an HLA determinant that includes specificities within, but not identical to, the B5 cross-reactive group. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 1193: Haynes BF, et al. Description of monoclonal antibody defining an HLA allotypic determinant that includes specificities within the B5 cross-reacting group. Hum. Immunol. 4: 273-285, 1982. PubMed: 6181035 | ||
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4D12
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