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- 2026年06月27日
企业认证
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- 相关疾病：
正常
- ATCC Number：
CCL-208™
- 器官来源：
肺
- 年限：
2 to 3 years
- 物种来源：
猕猴
- 是否是肿瘤细胞：
0
- 组织来源：
bronchus
- 细胞类型：
上皮细胞
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | 4MBr-5 | ||
| Depositors: | JL Caputo | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Macaca mulatta | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Tissue: bronchus Disease: normal Cell Type: epithelial |
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| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: May, 1978 | ||
| Receptors: | epidermal growth factor (EGF), expressed | ||
| Cytogenetic Analysis: | The stemline modal chromosome number is 45 occurring at 68%, and polyploidy at 1.6%. The only marker chromosome 14 pt is found in the majority (60%) of cells. N3 is tetrasomic and N19 is trisomic. There are two copies of X chromosomes, but no Y-like chromosome. The cell line appears to be of female origin. | ||
| Age: | 2 to 3 years | ||
| Comments: | This epithelial cell line was derived from the normal bronchial tissue of a 2- to 3-year-old rhesus monkey. The line was initiated in May, 1978 from tissue explants using glass chips. Cells contain PAS-positive cytoplasmic inclusions. The cells are positive for keratin by immunoperoxidase staining. |
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| Propagation: | ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: The line requires epidermal growth factor (EGF) for proliferation. |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 |
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| References: | 22657: . . In Vitro 15: 222-223, 1979. 88930: Rubin JS, et al. Purification and characterization of a newly identified growth factor specific for epithelial cells. Proc. Natl. Acad. Sci. USA 86: 802-806, 1989. PubMed: 2915979 |
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