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- 器官来源：
肾脏
- 相关疾病：
正常
- ATCC Number：
CRL-1586™
- 物种来源：
绿长尾猴
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 库存：
大量
- 细胞形态：
上皮样
- 运输方式：
冻存运输
| Designations: | VERO C1008 [Vero 76, clone E6, Vero E6] | ||
| Depositors: | EM Earley | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Cercopithecus aethiops | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | Plaques are also produced. VERO C1008 exhibits some degree of contact inhibition after forming a monolayer and is therefore useful in growing slow replicating viruses. When infected with the hemorrhagic fever viruses [Machupo (Bolivian), Junin (Argentinian), Lassa (African)], Marburg or Ebola viruses, these cells exhibit cytopathic effects. |
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| Virus Susceptibility: | Junin virus Machupo virus Lassa virus Marburg virus Zaire Ebola virus |
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| Comments: | This line is a clone of VERO 76 (ATCC CRL-1587 ). It was cloned by the dilution method into microtiter plates in 1979 by P.J. Price. When infected with the hemorrhagic fever viruses [Machupo (Bolivian), Junin (Argentinian), Lassa (African)], Marburg or Ebola viruses, these cells exhibit cytopathic effects. Plaques are also produced. VERO C1008 exhibits some degree of contact inhibition after forming a monolayer and is therefore useful in growing slow replicating viruses. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 22 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 26095: Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988 32579: Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020 |
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VERO C1008 [Vero 76, clone E6, Vero E6]
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