- 询价
- 2026年02月20日
企业认证
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 细胞形态：
上皮样
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 相关疾病：
肿瘤
- ATCC Number：
CRL-1655™
- 品系：
Wistar
- 器官来源：
膀胱
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | NBT-II [Nara Bladder Tumor No. 2] | ||
| Depositors: | J Leighton, R Tchao | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | epithelial |
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| Source: | Organ: urinary bladder Strain: Wistar Disease: tumor |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Gender: | male | ||
| Comments: | NBT-II cells differentiate in vitro giving rise to tumor giant cells. The differentiation is inhibited by vitamin A. The line was derived from a tumor which was induced with N-butyl-N-(4-hydroxybutyl) nitrosamine. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 22521: Toyoshima K, et al. Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(- 4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II. J. Natl. Cancer Inst. 47: 979-985, 1971. PubMed: 4941696 23029: Geisinger KR, et al. Appearance of giant cells as a morphological response of rat bladder carcinoma cell line (Nara Bladder Tumor No. 2) to a continuous thermal gradient in tissue culture. Cancer Res. 38: 1223-1230, 1978. PubMed: 639055 26131: Toyoshima K, Leighton J. Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. Cancer Res. 35: 1873-1879, 1975. PubMed: 1093679 26132: Tchao R. Novel forms of epithelial cell motility on collagen and on glass surfaces. Cell Motil. 2: 333-341, 1982. PubMed: 7151140 |
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plexus innervate the bladder. The parasympathetic nerve supply to the bladder controls urination. Increased sympathetic activity and angiotensin II constrict afferent and efferent arterioles, decreasing the capillary pressure. Because these changes
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