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- 细胞形态：
上皮样
- ATCC Number：
CRL-2872™
- 生长状态：
贴壁生长
- 是否是肿瘤细胞：
0
- 细胞类型：
上皮细胞
- 库存：
大量
- 运输方式：
冻存运输
- 器官来源：
皮肤
| Designations: | EPC [Epithelioma Papulosum Cyprini] | ||
| Depositors: | J Winton | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Morphology: | epithelial |
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| Source: | Organ: skin Cell Type: epithelial |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1969 | ||
| Virus Susceptibility: | Infectious pancreatic necrosis virus Infectious hematopoietic necrosis virus Spring Viremia of Carp Virus Viral hemorrhagic septicemia virus Epizootic hematopoietic necrosis virus Largemouth bass virus |
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| Comments: | The EPC line has a broad sensitivity for fish viruses. It is used internationally for isolation, propagation and diagnostic assays for fish viruses.. CRL-2872, originally deposited as Carp (Cyprinus carpio ), was subsequently found to be Fathead Minnow (Pimephales promelas ) through cytochrome c oxidase subunit I testing at ATCC . | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 25.0°C Max Temperature: 30.0°C Min Temperature: 20.0°C |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 sq. cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 |
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| Preservation: | Freeze medium: culture medium, 95%; DMSO,5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | about 36 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X Erythrosin B vital stain solution:ATCC 30-2404 Trypan Blue vital stain solution:ATCC 30-2402 |
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| References: | 92448: Fijan N, et al. Some properties of the Epithelioma Papulosum Cyprini (EPC) cell line from carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134 E: 207-220, 1983. 92449: Fryer JL, Lannan CN. Three decades of fish cell culture: A current listing of cell lines derived from fishes. J. Tissue Culture Methods 16: 87-94, 1994. 92450: Lorenzen E, et al. Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Dis Aquat Org 37: 81-88, 1999. PubMed: 10494498 92451: Loh PC, et al. Growth of the penaeid shrimp virus infectious hypodermal and hematopoietic necrosis virus in a fish cell line. J. Virol. Methods 28: 273-280, 1990. PubMed: 2117021 |
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EPC [Epithelioma Papulosum Cyprini]
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