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- ATCC Number：
CRL-2844™
- 品系：
Landrace
- 器官来源：
肺
- 生长状态：
贴壁生长
- 库存：
大量
- 物种来源：
猪
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 年限：
27 days
- 细胞形态：
单核细胞/巨噬细胞
- 运输方式：
冻存运输
| Designations: | 3D4/31 | ||
| Depositors: | J Gren | ||
| Biosafety Level: | 2 [cells containing SV40 viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Sus scrofa | ||
| Morphology: | macrophage |
||
| Source: | Organ: lung Strain: Landrace Cell Type: macrophage macrophage (alveolar); immortalized with SV40 large T antigentransformed with pSV3-neo |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: December, 1998 | ||
| Virus Susceptibility: | Bovine adenovirus 3 Classical swine fever virus Human parainfluenza virus 3 Swinepox virus Vesicular stomatitis New Jersey virus Porcine adenovirus Herpes simplex virus 1 African swine fever virus Pseudorabies virus Vaccinia virus Swine vesicular disease virus |
||
| Age: | 27 days | ||
| Gender: | unknown | ||
| Comments: | The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid . The plasmid carries the genes for neomycin resistance and SV40 large T antigen. Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845 ), 3D4/21 (ATCC CRL-2843 ) and 3D4/31 (ATCC CRL-2844 ). A subpopulation of each cell line was positive, to varying degrees depending on the media formulation, for nonspecific esterase activity and phagocytosis. Addition of DMSO to clone 3D4/31 upregulates the expression of CD14 monocyte marker. These porcine myelomonocytic cell lines may have a wide variety of applications in porcine virology and immunology [PubMed:12088830]. | ||
| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended Medium Renewal: Two to three times weekly |
||
| Preservation: | Freeze medium: Fetal bovine serum, 80%; complete growth medium, 10%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
||
| Doubling Time: | 19 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X Erythrosin B vital stain solution:ATCC 30-2404 Trypan Blue vital stain solution:ATCC 30-2402 MEM Non-Essential Amino Acid Solution, 100x:ATCC 30-2116 |
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| References: | 92770: Weingartl HM, et al. Continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility. J. Virol. Methods 104: 203-216, 2002. PubMed: 12088830 | ||
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