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- 文献和实验
- 技术资料
- ATCC Number：
CRL-1645™
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 库存：
大量
| Designations: | SJK-237-71 | ||
| Depositors: | D Korn | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human DNA polymerase alpha | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2db | ||
| Comments: | Mice were immunized with fraction VIII human DNA polymerase alpha. Spleen cells were fused with NS-1 myeloma cells. The antibody does not react with DNA polymerases beta and gamma, and does not neutralize polymerase alpha activity. Tested and found negative for ectromelia virus (mousepox). Formerly ATCC TIB-178. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X |
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| References: | 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672 | ||
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文献和实验200 216 225 230 234 237 239 241 242 244 245 246 247 248 1 2 18.5 19.0 19.2 19.2 19.3 19.3 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 2 3 10.1 9.55 9.28 9.12 9.01 8.94 8.89 8.85 8.81 8.79 8.74 8.71 8.69 8.67 8.66 3 4 7.71 6.94 6.59 6.39 6.26 6.16 6.09
ml 1000ml/ 82.03g/M 1M 2M 3M 4M Na(CH3 COO) [g] 82.03 164.06 246.09 328.12 H2 O [ml] 956.67 912.64 867.41 821.18 %(w/w) 7.897 15.237 22.101 28.550 H2 O %(w/w) 92.103 84.763 77.899 71.450 p (g/ml) 18o C
or RNA with DIG or Biotin 63 Estimating the Yield of DIG-labeled Nucleic Acids 65 Purification of labeled probes using the High Pure PCR Product Purification Kit 71 Chapter 5 Procedures for In Situ Hybridization to Chromosomes, Cells, and Tissue
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