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- 2026年06月19日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
- ATCC Number：
CRL-1861™
- 细胞形态：
上皮样
- 器官来源：
卵巢
- 运输方式：
冻存运输
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
- 生长状态：
混合型生长
| Designations: | EM9 (DNA repair mutant of CHO) | ||
| Depositors: | LH Thompson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed, adherent and suspension | ||
| Organism: | Cricetulus griseus | ||
| Morphology: | epithelial-like |
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| Source: | Organ: ovary | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Gender: | female | ||
| Comments: | This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61 ). EM9 is a repair deficient mutant derived from AA8 (see ATCC CRL-1859 ). The line was selected for enhanced sensitivity to ethylmethanesulfonate (EMS). The line is defective in single strand break repair, has a 10 fold higher baseline frequency of sister chromatid exchange relative to AA8 and a 2 fold greater sensitivity to killing by X-rays. This defect is corrected by the human XRCC1 gene. |
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| Propagation: | ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 1771: Thompson LH, et al. A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange. Mutat. Res. 95: 427-440, 1982. PubMed: 6889677 58396: Thompson LH, et al. A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391-405, 1980. PubMed: 7404270 |
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文献和实验Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene
Genetic screens have been extraordinarily useful for the identification of protein components that function in a variety of cellular processes. For example, a number of proteins that are necessary for responding to deoxyribonucleic acid (DNA
DNA修复 DNA repair 指双链 DNA上的损伤得到修复的现象。现在已知在生物体中有三种 DNA的修复：（ 1）光恢复修复：由紫外线所造成的 DNA损伤（胸腺嘧啶二聚体），由于光恢复酶的催化，在可见光照射后恢复成完整无损的状态．（ 2）切除修复：嘧啶二聚体或某种碱基损伤可以被切除．然后通过相对的一条完整无损的 DNA链作为模板重新进行合成，在这个被切除的部分中填上正常的碱基排列．在大肠杆菌中切除修复是由内切核酸修复酶， DNA多聚酶 I． DNA连接酶依次地进行作用的结果
Investigation of DNA Repair Pathway Activity
the ability of control or mutant cells to survive exposure to genotoxic agents that induce different types of DNA lesion. We also describe assays that assess the presence of markers for DNA repair within chromatin either in the form of posttranslational
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