- 询价
- 2025年10月12日
14 年金牌会员
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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 年限：
3 years
- 器官来源：
其他
- 细胞形态：
上皮样
- 库存：
大量
- 生长状态：
贴壁生长
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1903™
- 运输方式：
冻存运输
| Designations: | RC-4B/C | ||
| Depositors: | EH Leiter | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | epithelial |
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| Source: | Organ: pituitary, anterior Disease: adenoma |
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| Cellular Products: | luteinizing hormone beta (LH); growth hormone (GH); follicle stimulating hormone beta (FSH); prolactin; adrenal corticotropic hormone (ACTH); thyrotropin beta | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | gonadotropin releasing hormone (GnRH) | ||
| Age: | 3 years | ||
| Gender: | male | ||
| Comments: | This line was carried continuously in culture for 5 years before it was sucesssfully cryopreserved. The line contains cells producing LH, GH, FSH, prolactin, ACTH and thyrotropin beta (hormone production was tested using cells that were grown in medium containing Nu-serum rather than fetal bovine serum). GnRH receptors are about 2 fold lower in number than on normal pituitary. The cells contain numerous electron dense secretory granules, golgi complexes, and extended arrays of rough endoplasmic reticulum as well as endogenous rat C-type retrovirus particles. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 45%; alpha minimum essential medium with 1 g/L glucose, 45%; supplemented with 0.01 mM nonessential amino acids, 15 mM HEPES, 0.2 mg/ml bovine serum albumin, 2.5 ng/ml epidermal growth factor; dialyzed heat-inactivated fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| References: | 22681: Hurbain-Kosmath I, et al. Gonadotropes in a novel rat pituitary tumor cell line, RC-4B/C. Establishment and partial characterization of the cell line. In Vitro Cell. Dev. Biol. 26: 431-440, 1990. PubMed: 2161825 | ||
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RC-4B/C
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