![E.G7-OVA [derivative of EL4 (see ATCC TIB-39)]](https://custom.dxycdn.com/trademd/upload/pic/2012/03/29/1332499969.jpg!small.t.1)
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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 细胞类型：
T淋巴细胞
- 品系：
C57BL/6N
- 细胞形态：
淋巴样
- 库存：
大量
- 相关疾病：
淋巴瘤
- ATCC Number：
CRL-2113™
- 生长状态：
悬浮生长
| Designations: | E.G7-OVA [derivative of EL4 (see ATCC TIB-39)] | ||
| Depositors: | MJ Bevan | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Disease: lymphoma Strain: C57BL/6N Cell Type: T lymphocyte; |
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| Cellular Products: | chicken ovalbumin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2 b | ||
| Comments: | E.G7-OVA was derived in 1988 from the C57BL/6 (H-2 b) mouse lymphoma cell line EL4 (ATCC TIB-39 ). The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene. E.G7-OVA cells contain a single copy of the inserted plasmid and synthesize and secrete OVA constitutively. C57BL/6 mice immunized with E.G7-OVA cells give rise to H-2 Kb restricted cytotoxic lymphocytes specific for the OVA 258-276 peptide. This cell line is a model system for studying major histocompatibility complex (MHC) class I restricted responses of cytotoxic T lymphocytes in mice. |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 0.4 mg/ml G418, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC TIB-39 |
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| References: | 22630: Moore MW, et al. Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777-785, 1988. PubMed: 3261634 | ||
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E.G7-OVA [derivative of EL4 (see ATCC TIB-39)]
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