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- ATCC Number：
CRL-1968™
- 运输方式：
冻存运输
- 免疫类型：
IgG
- 细胞形态：
淋巴样
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 生长状态：
悬浮生长
| Designations: | UC8-1B9 | ||
| Depositors: | JA Bluestone | ||
| Isotype: | IgG | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with DNP derivatized KLH (keyhole limpet hemocyanin). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas. |
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| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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