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- ATCC Number：
CRL-2196™
- 年限：
6 weeks
- 运输方式：
冻存运输
- 品系：
BALB/c
- 库存：
大量
- 生长状态：
贴壁生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 细胞形态：
上皮样
| Designations: | GC-2spd(ts) | ||
| Depositors: | MC Hofmann, J Millan | ||
| Biosafety Level: | 2 [CELLS CONTAIN PAPOVAVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial |
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| Source: | Cell Type: spermatocyte; SV40 large T antigen transfected Strain: BALB/c |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 6 weeks | ||
| Gender: | male | ||
| Comments: | GC-2spd(ts) was established by stable cotransfection of freshly isolated spermatocytes with the SV40 large T antigen gene (pSV3neo, see ATCC 37150 ) and a temperature sensitive mutant of the p53 tumor suppressor gene (LTRp53cG9). Cells were selected with G-418, cultivated for 6 months and single cell cloned three times by limiting dilution. No clonal proliferation was observed in soft agar cultures, indicating that these cells were immortalized but not transformed. The cells have lost their differentiation potential, and are currently arrested at a premeiotic stage. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:7 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 22715: Hofmann MC, et al. A haploid and a diploid cell coexist in an in vitro immortalized spermatogenic cell line. Dev. Genet. 16: 119-127, 1995. PubMed: 7736662 23344: Hofmann MC, et al. Immortalized germ cells undergo meiosis in vitro. Proc. Natl. Acad. Sci. USA 91: 5533-5537, 1994. PubMed: 8202522 |
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文献和实验ES and TS cell freezing/thawing
Needed: ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be used, and should comprise freshly prepared 60% DMEM+, 20% FCS, and 20% DMSO (Sigma, Cat. No.D-5879). Freezing
凯氏点 Casparian dots，Casparian poin-ts，radial dots
亦称凯氏带（Casparian strips）。是指从上下左右四个侧面围着植物内皮层细胞的带状肥厚物，它是内皮层细胞的特征之一。从横切面看，在左右两侧细胞壁的中央部位呈点状物，但其大小和形状是各种各样的。它已角质化或木栓质化。在凯氏点部分，原生质膜的性质有一些不同，即使在其他部分发生原生质可出现分离，而凯氏点部分的原生质膜不分离。
ES and TS cell freezing/thawing
ES and TS cell freezing/thawing Needed: ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be used, and should comprise freshly prepared 60% DMEM+, 20% FCS, and 20% DMSO (Sigma
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