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- 2026年03月22日
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- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- ATCC Number：
HB-9571™
- 库存：
大量
| Designations: | 18C2.8.3 | ||
| Depositors: | Univ. Georgia Res. Fndn. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against NK cell target ligand on NC-37 cells | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with NC-37 (see ATCC CCL-214 ) cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with NC-37 cells and inhibits NK cell mediated lysis. The antibody also blocks lysis by catfish nonspecific cytotoxic cells (NCC) which are analogous to mammalian NK cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22020: Evans DL. Antibodies to natural killer cell and non-specific cytotoxic cell receptor and target cell antigens. US Patent 5,194,593 dated Mar 16 1993 | ||
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