- 询价
- 2026年03月22日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- ATCC Number：
CRL-2434™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- 生长状态：
混合型生长
| Designations: | 1G3 | ||
| Depositors: | PJ Bjorkman | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed: adherent and suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). The antibody recognizes FcRn heavy chain heterodimers. FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules. The IG3 (CRL-2434) antibody recognizes two lipid linked forms of FcRn. These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin). It also recognizes the FcRn-DAF construct. The 2G3 (CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct. Unlike 4C9 (CRL-2437), the monoclonal antibodies IG3 (CRL-2434)and 2G3 (CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains. This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. [39787 ] |
||
| Propagation: | ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201). Temperature: 37.0°C |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 - 4 day is recommended Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml. |
||
| Preservation: | HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%. | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 39787: Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
设备：1. 分光光度计；2. 离心机；3. 恒温水浴（37℃，70℃，100℃）；4.具塞刻度试管；5. 刻度吸管；6. 容量瓶。（三）试剂（均为分析纯）：1. 标准麦芽糖溶液（1mg/ml）：精确称取100mg麦芽糖，用蒸馏水溶解并定容至100ml；2. 3，5－二硝基水杨酸试剂：精确称取1g3，5－二硝基水杨酸，溶于20ml2mol/L NaOH溶液中，加入50ml蒸馏水，再加入30g酒石酸钾钠，待溶解后用蒸馏水定容至100ml。盖紧瓶塞，勿使CO2进入。若溶液混浊可过滤后使用；3.01mol/L pH
4Iam9KioaEuNjpt2rSpQuy9e/eO5Iz5D7t27iQCsESYV4jUNzWICR9KcLkVVftpk5wuzs7OSrCCr4cFvhY/15s1XZuI3KjoN0discgzxqRthRdd1wHwMDmM/WSrxT98EHoG78VFogkwGeiJuvklJM6IwvlZ47ArFND18MbSgS1g3Ho+/Nv/jhsnf8H0NrWx+Xvb7K0FNZNG6Dd6OCKldvH4lpLYGBzi7/pgP6FFJATc+us33MqH7diTPrg
技术资料暂无技术资料 索取技术资料
1G3
询价
