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- 2026年07月09日
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- 运输方式：
冻存运输
- 品系：
DBA/2
- 相关疾病：
淋巴瘤
- ATCC Number：
CRL-9518™
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 生长状态：
悬浮生长
| Designations: | L5178Y TK+/- clone (3.7.2C) [TK+/- (clone 3.7.2C)] | ||
| Depositors: | Mobil Oil Corporation | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Disease: lymphoma Strain: DBA/2 |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | This line is a derivative of L5178Y-S (ATCC CRL-1723 ). | ||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1% Pluronic, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 3 to 4 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 21875: . . Chem. Mutagens 3: pp. 79-103, 1973. | ||
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文献和实验|998894|gb|S78179.1|S78179 Oryctolagus cuniculus type XII... 32 0.037 gi|31072033|gb|AC122153.4| Oryctolagus cuniculus clone LB1-... 28 0.58 gi|1496|emb|X52496.1|OCCAATP Rabbit mRNA for calcium ATPase 28 0.58 gi|1468|emb|X02814.1|OCATPDR Rabbit mRNA
Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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