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- 年限：
3 months
- 器官来源：
肝
- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- 品系：
CD-1
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 运输方式：
冻存运输
- 相关疾病：
正常
- ATCC Number：
CRL-2254™
| Designations: | AML12 | ||
| Depositors: | N Fausto | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Strain: CD-1 Disease: normal Cell Type: hepatocyte; |
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| Cellular Products: | albumin; human transforming growth factor alpha (TGF alpha); mouse TGF alpha | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Tumorigenic: | No | ||
| Age: | 3 months | ||
| Gender: | male | ||
| Comments: | The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha. By electron microscopy, these cells exhibit typical hepatocyte features such as peroxisomes and bile canalicular like structure. AML12 cells retain the capacity to express high levels of mRNA for serum (albumin, alpha 1 antitrypsin and transferrin) and gap junction (connexins 26 and 32) proteins, and contain solely isoenzyme 5 of lactate dehydrogenase. The cells express high levels of human TGF alpha and lower levels of mouse TGF alpha. Expression of liver specific proteins decreases with time in culture, but is reactivated by growing the cells in serum free medium. |
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| Propagation: | ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 0.005 mg/ml insulin, 0.005 mg/ml transferrin, 5 ng/ml selenium, and 40 ng/ml dexamethasone, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 37 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 |
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| References: | 22600: Wu JC, et al. Establishment and characterization of differentiated, nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha. Proc. Natl. Acad. Sci. USA 91: 674-678, 1994. PubMed: 7904757 23390: Dumenco L, et al. Introduction of a murine p53 mutation corresponding to human codon 249 into a murine hepatocyte cell line results in growth advantage, but not in transformation. Hepatology 22: 1279-1288, 1995. PubMed: 7557882 |
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的 DnaJB1-PKA 融合蛋白的确会破坏细胞内 RIα 的液 - 液相分离，进而使得 cAMP/PKA 信号通路失调。也就是说 DnaJB1-PKAcat 对 RIα 的液 - 液相分离的破坏显著损害了 cAMP 的区隔化，为癌蛋白融合引起的异常信号传导提供了机制线索。 为了测试缺失 RIα 的液 - 液相分离功能的后果，研究使用非致瘤性肝细胞 AML12，在该细胞系中稳定表达了野生型或各种突变型的 RIα，通过测量它们的增殖率和转化能力，研究发现与野生型细胞相比，缺失 RIα 可使细胞增殖和 DNA
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AML12
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