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- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 相关疾病：
其他疾病
- 运输方式：
冻存运输
- ATCC Number：
CRL-9589™
- 细胞类型：
其他细胞类型
- 库存：
大量
- 细胞形态：
淋巴样
- 年限：
13 years
- 器官来源：
外周血
- 生长状态：
悬浮生长
| Designations: | AML-193 | ||
| Depositors: | Wistar Institute | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Disease: acute monocytic leukemia Cell Type: monocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | CD15, human; Homo sapiens, expressed (75 to 95% of the cells) (75 to 95% of the cells) | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 12 D5S818: 9,13 D7S820: 9,12 THO1: 7,9 TPOX: 8,9 vWA: 15,17 |
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| Cytogenetic Analysis: | This is a hyperdiploid human cell line. The modal chromosome number is 49, occurring in 50% of cells. The rate of polyploidy is 2.2%. There is one marker chromosome, der(17)t(17;17)(p13.1;q21.3), that occurred in every cell. These cells are trisomic for N3, N6, N8, and N13, and occasionally also for N2. N17 and X chromosome had a single copy, but occasionally the X had two copies. Thus the modal karyotype of this cell line is 49, X, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3), -17. The subline with 50, X, +2, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3), -17 and 50, XX, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3, -17 also occurred, but at a very low frequency. | ||
| Age: | 13 years | ||
| Gender: | female | ||
| Comments: | Interleukin-3 (interleukin 3, IL-3) and granulocyte/macrophage colony stimulating factor (GM-CSF) act synergistically to stimulate growth of the cells. Granulocyte colony stimulating factor (G-CSF) also supports short term and long term growth of AML-193 cells and acts synergistically with GM-CSF in inducing proliferation of the cells.This line was formerly designated ATCC HTB-188. | ||
| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium with 0.005 mg/ml insulin, 0.005 mg/ml transferrin and 5 ng/ml GM-CSF, 95%; fetal bovine serum, 5%. Temperature: 37.0°C Growth Conditions: The cells are dependent upon GM-CSF for long term growth. Conditioned medium from U-87MG (ATCC HTB-14 ) may be used at a concentration of 10% to replace the GM-CSF in the medium (see J. Immunol. 139:3348- 3354, 1987). |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 3 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
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| References: | 22617: Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132 23467: Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218 |
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文献和实验AML Sample genomic DNA Preparation
Solutions PBS Cell lysis buffer: 10 mM Tris pH8, 100 mM NaCl, 25 mM EDTA, 0.5% (w/v) SDS, 0.5 mg/mL proteinase K Fixative: 1 volume glacial acetic acid + 3 volumes methanol Trizol reagent 75% EtOH made with RNAse free H2 O 100
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网络 得乐治疗 慢性胃炎 193例 1984的Marshall成功地从胃炎病人的胃内分离出了幽门螺杆菌（Hp），以后又对Hp的形态理化特点及与胃炎的发病作了大量的研究，从而揭示出Hp与胃炎的相关性因素。经研究Hp常寄生在胃窦部向胃体部蔓延，组织学检查多见于胃液深层胃表面上皮游离面，两个表面的上皮
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