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- 详细信息
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 免疫类型：
mouse IgG2a
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
- ATCC Number：
HB-163™
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | AF6-120.1.2 | ||
| Depositors: | AM Stall | ||
| Isotype: | mouse IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against I-A b | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with spleen cells from C57BL/10J mice. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with cells bearing the I-A of the b, k and u H-2 haplotypes. The antibody does not react with I-A of the d, f, q, r or s H-2 haplotypes. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Interval: Maintain cultures at a cell concentraton between 5 X 10(4) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 1204: Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J. Immunol. Methods 50: R85-R112, 1982. PubMed: 7047643 | ||
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文献和实验P＝0.05 （双侧） r r（较大均方的自由度） n 2 2 3 4 5 6 7 8 9 10 12 15 20 30 60 00 1 799 364 899 922 937 948 957 963 969 977 985 993 1001 1010 1018 1 2 39.0 39.2 39.2 39.3 39.3 39.3 39.4 39.4 39.4 39.4 39.4 39.4 39.5 39.5 39.5 2 3 10.0 15.4 15
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获得异种移植物。满足 CDCscreen score≥2、有效 gRNAs≥2 且倍数变化≤0.5 条件的共有 79 个 circRNA，其中 8 个与 HT29、HeLa、293FT 及 H9 细胞系中进行功能缺失（loss-of-function，LOF）表型体外筛选结果重叠。CircFAM120A 是这些重叠的候选基因之一。上述结果验证了 RfxCas13d/BSJ-gRNA 可用于人细胞系功能性 circRNA 筛选。 CircFAM120A 的体内体外筛选（图源：Nature
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