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- 2026年03月22日
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- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 器官来源：
卵巢
- ATCC Number：
CRL-9010™
- 运输方式：
冻存运输
| Designations: | DUKX B1 [5A HS MYC] | ||
| Depositors: | R Kingston | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent, adherent | ||
| Organism: | Cricetulus griseus | ||
| Morphology: | epithelial |
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| Source: | Organ: ovary | ||
| Cellular Products: | over produces mouse c-myc protein when heated | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Gender: | female | ||
| Comments: | The cells are reported to contain DNA sequences from mouse, drosophila, adenovirus and E. coli. | ||
| Propagation: | ATCC complete growth medium: Alpha modified Eagle's minimum essential medium with 0.32 mM methotrexate, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37�C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: culture medium ,95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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sapiens synaptogyrin 2 (SYNGR2), mRNA 1414 NM_001127 Homo sapiens adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 1, mRNA 873 NM_002107 Homo sapiens H3 histone, family 3A (H3F3A), mRNA 9328 NM_003899 Homo sapiens Rho
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DUKX B1 [5A HS MYC]
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