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- 细胞形态：
上皮样
- 年限：
fawn
- 相关疾病：
正常
- 运输方式：
瓶装运输
- ATCC Number：
CRL-6194™
- 器官来源：
肝
- 库存：
大量
- 生长状态：
贴壁生长
- 物种来源：
其他
- 是否是肿瘤细胞：
0
| Designations: | OHH1.Li | ||
| Biosafety Level: | 1 | ||
| Shipped: | flask | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Odocoileus hemionus hemionus | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Cytogenetic Analysis: | modal number = 70; range = 69 to 71; 1 large and 2 medium metacentrics | ||
| Age: | fawn | ||
| Gender: | male | ||
| Comments: | Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. Note: This item is distributed only within the 50 United States. It is not available for international distribution. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-30-2002 Recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Phosphate-buffered saline: ATCC 30-2200 Cell culture tested DMSO: ATCC 4-X |
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文献和实验TP53, hChk2, and the Li-Fraumeni Syndrome
In 1969 Li and Fraumeni (1 ,2 ) reported a systematic epidemiologic study of the incidence of cancers in relatives of children with rhabdomyosarcoma (RMS). They noted five families of 648 probands in which a sib or cousin was also affected
Mass Spectrometry Analysis of Polyisoprenoids Alcohols and Carotenoids via ESI(Li+)-MS/MS
-MS approach for the direct analysis of polyisoprenoid alcohols from biological samples. Lithium iodide is used to promote cationization by intense formation of [M+Li]+ adducts. Detection of polyisoprenoids with mass determination can thus be performed
一.测定1.把蓄电池接到2个蓄电池插孔中的任一个，干燥管中换入新的干燥剂，打开电源开关，显示器将闪烁。接着瞬息指示“Pad Cleared”(页已清除)，然后显示软件版本“LI—6200.02.00”2.检查蓄电池电压与内存容量(按U键)。3.检查页参数(按SETU或FCT41)。4.检查时钟和校正表(按FCT43：时钟，FCT44：量子传感器；FCT45：C02分析器；FCT46：流量计；FCT47：湿度传感器。5.流量计凋零（按FCT48）。6.设置分析器参数（按FCT49）。7.设置LI
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