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- 2025年11月09日
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- 技术资料
- 细胞形态：
上皮样
- 年限：
adult
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 库存：
大量
- 生长状态：
贴壁生长
- 器官来源：
肾脏
- 运输方式：
冻存运输
- 相关疾病：
正常
- ATCC Number：
CCL-56™
| Designations: | PtK2 (NBL-5) | ||
| Depositors: | KH Whalen | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Potorous tridactylis | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Disease: normal |
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| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Virus Resistance: | adenovirus 5; coxsackievirus B5; poliovirus 2 | ||
| Age: | adult | ||
| Gender: | male | ||
| Comments: | The cells are positive for keratin by immunoperoxidase staining. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 daysEvery 2 to 3 days |
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| Preservation: | Freeze medium: growth medium, 95%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 26208: . . Genetics 51: 915, 1965. | ||
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PtK2 (NBL-5)
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